液中で動作する周波数変調原子間力顕微鏡（FM-AFM）について、その動作原理および構成を概説し、分子分解能での生体分子の構造観察事例を紹介する。さらに、フォースマッピング法によって固液界面での相互作用力分布を取得することで、水和・溶媒和計測や電荷分布計測が可能となる。いくつかの固液界面物性計測事例について紹介したい。一方、AFMで得られる表面物性は通常物理的な性質に限られるが、AFMとラマン分光を組み合わせた探針増強ラマン分光（TERS）によれば、化学的な性質も評価することが可能となる。講演では、薄膜導波路構造を有するプローブを用いた高感度TERS装置の開発状況および展望について述べたい。
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